Inclusion Criteria:

– Skeletally mature subjects at least 25 years of age

– Chronic lower back pain for at least 6 months

– Failure to respond to at least 6 months of non-operative conservative management

Exclusion Criteria:

– Radicular pain

– Current or history of spinal infection

– Modic changes at vertebral bodies other than L3 to S1

– Contraindication to MRI

– Pregnant, lactating or plan to become pregnant in next year

– Has life expectancy of less than 2 years

– Compensated injuries or ongoing litigation regarding back pain/injury, or financial
incentive to remain impaired

Inclusion Criteria:

1. Male or female, between 18 and 75 years of age, inclusive, and skeletally mature, in
the opinion of the investigator.

2. Has provided written informed consent before undergoing any study-specific procedures.

3. Has chronic low back pain for at least 6 months, where back pain is greater than leg
pain.

4. Has separate low back pain and leg pain VAS scores within the following parameters:

1. Average low back pain VAS score of at least 40 mm and less than 90 mm on a 100 mm
visual analog scale.

2. Average leg pain VAS score that is less than or equal to 50% of the average low
back pain VAS score above, where leg pain VAS scores are measured on a 100 mm
visual analog scale.

5. Has had inadequate response to conservative medical care over a period of at least 3
months.

6. Diagnosis of painful degenerative disc disease at one lumbar level from L1 to S1
confirmed by subject history and radiographic studies (eg, MRI, x-rays). Radiographic
studies must demonstrate the following:

1. A Pfirrmann score of 3 or 4 on MRI in just a single disc between L1 and S1

2. With or without contained disc herniations of <3 mm protrusion with no radiographic evidence of neurologic compression 3. Disc height loss of the symptomatic disc is less than 50% of the adjacent discs 4. All discs other than the index disc must have a Pfirrman score of less than 3 5. No spondylolisthesis or instability on flexion/extension views of more than 1-2 mm 6. No evidence of sacroiliac, or extra-spinal, pathology that could account for lumbar back pain 7. No more fluid in, or widening of, the facet joints around the symptomatic disc than is seen on the other facet joints of "normal" levels 7. Has a Body Mass Index (BMI) between 15.0 and 35.0 kg/m2 at the screening visit. 8. Oswestry Disability Index score of at least 30 and not more than 90 on the 100 point questionnaire. 9. Willing and able to comply with all protocol requirements. 10. Females of childbearing potential must agree to use highly effective methods of contraception during heterosexual intercourse from the date they sign the ICF until 1 year after the single dose of study drug is administered. 11. Male subjects must agree that, if their partner is of childbearing potential, they and the partner will use an appropriate method of contraception as defined above. Exclusion Criteria: 1. Has any prior or current medical condition (eg, an active systemic infection), including known bleeding disorders that, in the judgment of the Investigator, would prevent the subject from safely participating in and/or completing all study requirements. 2. Has any clinically significant finding on a screening evaluation that in the judgment of the Investigator would preclude safe study completion. 3. Liver function tests >1.5 the upper limit of normal (ULN) for alkaline phosphatase,
alanine aminotransferase (ALT), aspartate aminotransferase (AST)

4. Serum creatinine >1.5 ULN at screening

5. Blood pressure >160/110 mm Hg recorded on 3 readings at screening and/or resting pulse
>120 beats/min at screening

6. Body temperature ≥101°F on day of planned injection

7. Has a co-morbid medical condition of the spine or the upper extremities that may
affect neurological and/or pain assessments as specified in the protocol, including
spinal fusion, spondylolysis and spondylolisthesis.

8. Has evidence of hip pathology based on clinical history, physical exam and/or
radiographic imaging that could be the source of lumbar back pain.

9. Has a history of an endocrine or metabolic disorder that affects the spine (eg,
Paget’s disease).

10. Has a compressive pathology due to stenosis or frankly herniated disc or sequestered
discs.

11. Has symptomatic involvement of more than one lumbar disc, in the opinion of the
investigator.

12. Has an intact disc bulge/protrusion or focal herniation at the symptomatic level >3
mm, the presence of disc extrusion or sequestration, or a complete annular tear.

13. Has lumbar intervertebral foraminal stenosis at the symptomatic level resulting in
clinically significant spinal nerve root compression, in the opinion of the
investigator.

14. Has undergone a previous surgery at the symptomatic disc, or has plans to do so within
6 months of receiving the dose of AMG0103, that has altered the structure of the
target disc level, or is likely to do so (eg, laminectomy, foraminotomy, fusion,
intradiscal electrothermal therapy, intradiscal radiofrequency, thermocoagulation).

15. Has had any lumbar intradiscal injection procedure (eg, injection of corticosteroids,
methylene blue, dextrose, or glucosamine and chondroitin sulfate). Discography may be
performed, but must be done at least 3 weeks (or more) prior to the injection
procedure in this study.

16. Has had any epidural steroid injection(s) within 3 months prior to study treatment.

17. Is a female who is pregnant, planning to become pregnant, or lactating.

18. Has a PT(INR) and/or aPTT lab result outside of the reference range, or within the
last 30 days taken, or is currently on, an anticoagulant therapy (e.g., Warfarin) for
the purpose of treating or preventing blood clots.

19. Has ferromagnetic implants that would disallow MRI of the symptomatic disc.

20. Is involved in current or pending spinal litigation where permanent disability
benefits are being sought.

21. Has a physical or mental condition (eg, senile dementia, Alzheimer’s disease) that
would interfere with Subject self-assessment of function, pain or quality of life.

22. Has a positive screen for Hepatitis B Surface Antigen, Hepatitis C antibodies, or
human immunodeficiency virus (HIV) by antibodies or nucleic acid test.

23. Is an immediate family member (by birth) of any other subject participating in this
study.

24. Has a history of cancer that does NOT have documentation to support a complete and
curative treatment.

25. Has participated within the previous 3 months in an interventional clinical study or
is concurrently enrolled in any non-interventional research of any type judged to be
scientifically or medically incompatible with this study.

26. Is transient or has been treated in the 6 months prior to providing informed consent
for alcohol and/or drug abuse in an inpatient substance abuse program.

27. Is currently incarcerated (a prisoner).

28. Is an Investigator-site employee or immediate family (defined as a spouse, parent,
child, or sibling, whether biological or legally adopted) of an Investigator-site
employee.

29. Is a Sponsor employee (permanent, temporary contract worker, or designee responsible
for the conduct of the study) or immediate family (defined as a spouse, parent, child,
or sibling, whether biological or legally adopted) of a Sponsor employee.

Inclusion Criteria: The subject must have:

1. Diagnosis of early to moderate degenerative disc disease (DDD), Modified Pfirrmann
Grade 3-7.

2. Chronic low‑back pain for at least 6 months prior to screening; unresponsive to at
least 3 months of conservative care.

3. Low‑back pain of 40 to 90 mm on the VAS and ODI score of 30 to 90.

Exclusion Criteria: The subject is excluded if he/she has:

1. Symptomatic involvement of more than one lumbar disc.

2. Other persistent pain/nerve issues including, for example, radiculopathy, leg pain,
cauda equine syndrome, etc.

3. Fracture of the spine, previous lumbar spine surgery or previous treatment of the
target disc.

4. Evidence of dynamic instability on lumbar flexion‑extension radiographs.

5. Grade 2 or higher spondylolisthesis at the target disc, lumbar spondylitis or other
undifferentiated spondyloarthropathy, or Type III Modic changes around the target
disc.

6. Clinical suspicion of a full thickness annular tear at the target disc or other
abnormal disc morphology.

7. Clinical suspicion of facet pain as primary pain generator.

8. Subjects who test positive for communicable disease, have significant systemic
disease, or are prone to infection.

9. Patients who are deemed unsuitable for clinical study participation by the
investigator.

Inclusion Criteria:

– Subject is 18 years of age or older

– Subject had a spinal procedure where a dural opening (either intentional or
incidental) occurred and was treated with either:

– DuraSeal™ Exact Spine Sealant (DuraSeal™ Sealant group ONLY) OR

– Any other method of sealing the dura with the exception of DuraSeal™ Sealant
-either spinal or cranial. (Control group ONLY)

– Subject, or authorized representative, has been informed of the nature of the study
and has provided written informed consent approved by the appropriate Institutional
Review Board (IRB) of the respective clinical site prior to the collection of study
data.

– Prospective subjects: Consent must be obtained within 24 hours of surgery stop
time.

– Retrospective subjects (Control group specific): IRB approval may be granted to
individual sites to waive requirement for informed consent.

Exclusion Criteria:

– The investigator determines that the subject will not be able to comply with the
required follow-up visits (not required if subject is being enrolled retrospectively-
control group ONLY)

– Pregnant or breastfeeding females (as documented in the medical records; no testing
beyond the site’s standard of care is required)

Inclusion Criteria:

– A subject may be included if s/he meets the following criteria:

1. ≥18 years of age;

2. Able to give appropriate informed consent;

3. Willing and able to meet the proposed follow-up schedule;

4. Has documented diagnosis of up to, and including, Grade 1 spondylolisthesis
and/or degenerative disc disease;

5. Has given appropriate consent for, and undergoes, standard-of-care transforminal
or posterior lumber interbody fusion;

6. Use of InQu Bone Graft Extender on-label.

Exclusion Criteria:

1. Any prior lumbar fusion surgery;

2. Requires fusion surgery of more than two adjacent levels;

3. Has fusion surgery requiring titanium cages;

4. Has a systemic infection or has infection at the site of surgery;

5. Has a medical condition or requires post-operative medication that, in the opinion of
the investigator, may interfere with bony/soft tissue healing;

6. Has any physical, social, psychological or economic condition that, in the opinion of
the investigator, may preclude accurate data collection or evaluation.

Key Inclusion Criteria:

– Aged 16 – 70 years

– AIS A traumatic spinal cord injury at neurological spinal cord level T2-T12/L1

– Recent injury (must receive Scaffold within 96 hours from injury)

– Non-penetrating contusion injury no less than approximately 4 mm diameter by MRI

Key Exclusion Criteria:

– Incomplete spinal cord injury (AIS B, C, D, E)

– Terminally ill

– Spinal cord injury associated with traumatic brain injury

– Subject on long term mechanical ventilation

– Penetrating injuries

– Radiographic or visual evidence of parenchymal dissociation or anatomic transection
where the contusion completely bridges a full cross-section of the spinal cord

Inclusion Criteria:

– Subject must be scheduled to undergo an elective open posterior spinal fusion
procedures with multilevel instrumentation, 10 to 60 days after study vaccination.

– Subject must be available for the entire duration of the study, and able to comply
with scheduled visits, treatment plan, laboratory tests, and other study procedures
including completion of the electronic diary for 10 days after study vaccination.

– Aged 18 to <86 years old Exclusion Criteria: - Planned spinal fusion procedure requiring separate operations performed on separate days (ie, staged procedure). - Surgical indication of malignancy, infection or acute or emergency trauma. - History of major surgery within 3 months prior to enrollment, or anticipated major surgery other than the Index Surgical Procedure between study enrollment and completion of study participation. - History of any spinal surgery performed within 6 months prior to study enrollment. - History of any previous spinal surgery resulting in postoperative BSI or SSI. - Congenital or acquired immunodeficiency disorder, rheumatologic disorder or other illness requiring chronic treatment with known immunosuppressant medications, including monoclonal antibodies within a year of enrollment or the use of systemic corticosteroids for > 14 days within 30 days prior to enrollment.

– History of leukemia, lymphoma, underlying bone marrow disorder or history of bone
marrow transplant.

Major Inclusion Criteria:

– Sensorimotor complete, traumatic SCI (ASIA Impairment Scale A) for cohorts 1,2,3

– Sensorimotor incomplete, traumatic SCI (ASIA Impairment Scale B) for cohorts 4,5

– Last fully preserved single neurological level (SNL) from C-4 to C-7

– From 18 through 69 years of age at time of injury

– Single spinal cord lesion on a post-stabilization magnetic resonance imaging (MRI)
scan, with sufficient visualization of the spinal cord injury epicenter and lesion
margins to enable post-injection safety monitoring

– Informed consent for this protocol and the companion long term follow-up protocol must
be provided and documented (i.e., signed informed consent forms) no later than 37 days
following injury

– Able to participate in an elective surgical procedure to inject AST-OPC1 21-42 days
following SCI

Major Exclusion Criteria:

– SCI due to penetrating trauma

– Traumatic anatomical transection or laceration of the spinal cord based on prior
surgery or MRI

– Any concomitant injury that interferes with the performance, interpretation or
validity of neurological examinations

– Inability to communicate effectively with neurological examiner such that the validity
of patient data could be compromised

– Significant organ damage or systemic disease that would create an unacceptable risk
for surgery or immunosuppression

– History of any malignancy (except non-melanoma skin cancers)

– Pregnant or nursing women

– Body mass index (BMI) > 35 or weight > 300 lbs.

– Active participation in another experimental procedure/intervention